人源WNT-5a 蛋白因子,5ug 保存方法 The lyophilized protein is stable for at least 6 months if stored at -80 degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.
產品英文描述 WNT-5a belongs to the class of WNT proteins that activate the “noncanonical” pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product WNT-5a,human W5A-H-005,5ugis expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method. ?WNT-5a,human W5A-H-005,5ug Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis ?WNT-5a,human W5A-H-005,5ugBiological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparat